Scientific Image Processing and Analysis

This two-day course on basics in processing and analysis of digital images according to high scientific standards covers:

• Basics in microscopy, good image acquisition, resolution and sampling
• Correct resizing, contrast improvement and labeling
• Reducing background, and the use of basic image filters
• Application of image filters for post-processing
• Feature (cells, objects) extraction by segmentation with thresholding
• Automatic object counting and measuring
• Measurements and quantifications of fluorescence intensities (optional: Western blots)
• ƒoptional: Analysis of brightfield / color images (e.g. photographs)
• Working with image stacks and higher dimensional images
• ƒƒFigure preparation guideline (including good scientific practice and ethics)
• Batch processing, automation of your analyses (using macros)
  

ƒƒThe workshop has a focus on life science applications and microscopic images. We will use the professional software ImageJ / Fiji for processing and analysis. Prior knowledge of the software is not required.